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Janssen eeg power spectra
Eeg Power Spectra, supplied by Janssen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eeg+power+spectra/eeg+power+spectra/pm27728831-197-35-55
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Article Title: On-going electroencephalographic rhythms related to cortical arousal in wild-type mice: the effect of aging.
Article Snippet: Please cite this article as: Del Percio, C., Drinkenburg, W., Lopez, S., Infarinato, F., Bastlund, J.F., Clausen, B., Pedersen, J.T., Christensen, D.Z., Forloni, G., Frasca, A., Noè, F.M., Bentivoglio, M., Fabene, P.F., Bertini, G., Colavito, V., Kelley, J., Dix, S., Richardson, J.C., Babiloni, C., On behalf of PharmaCog Consortium, On-Going Electroencephalographic Rhythms Related to Cortical Arousal in Wild Type Mice: the Effect of Aging, Neurobiology of Aging (2016), doi: 10.1016/ j.neurobiolaging.2016.09.004.



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Chemogenetic activation of MPB (but not LPB) neurons induces wakefulness. (A,B) Schematic diagram indicating the position of hM3Dq-mCherry protein was confined to the MPB (A) or LPB (B) . Superimposed mCherry expression areas in the MPB (A) and LPB (B) of 8 AAV-injected rats in each group, are shown in the right panels . Scale bars: 1 mm. (C,D) Typical examples of <t>EEG</t> <t>power</t> spectra, EEG, EMG, hypnogram, and relative magnitude changes in delta and theta power over 24 h for a rat expressing hM3Dq at MPB (C) or LPB neurons (D) when given CNO (0.3 mg/kg). (E,F) Time courses of wakefulness after administration of CNO (0.3 mg/kg) or saline in rats expressing hM3Dq in MPB (E) or LPB neurons (F) . The arrow indicates the time point of CNO or saline administration, and open and closed bars above the x -axis indicate light and dark periods, respectively. (G,H) EEG Power spectrum of wakefulness during 10 h (9:00–19:00) after the CNO injection in MPB-hM3Dq (G) and LPB-hM3Dq rats (H) . * P < 0.05, ** P < 0.01, compared with saline ( n = 8).
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Chemogenetic activation of MPB (but not LPB) neurons induces wakefulness. (A,B) Schematic diagram indicating the position of hM3Dq-mCherry protein was confined to the MPB (A) or LPB (B) . Superimposed mCherry expression areas in the MPB (A) and LPB (B) of 8 AAV-injected rats in each group, are shown in the right panels . Scale bars: 1 mm. (C,D) Typical examples of <t>EEG</t> <t>power</t> spectra, EEG, EMG, hypnogram, and relative magnitude changes in delta and theta power over 24 h for a rat expressing hM3Dq at MPB (C) or LPB neurons (D) when given CNO (0.3 mg/kg). (E,F) Time courses of wakefulness after administration of CNO (0.3 mg/kg) or saline in rats expressing hM3Dq in MPB (E) or LPB neurons (F) . The arrow indicates the time point of CNO or saline administration, and open and closed bars above the x -axis indicate light and dark periods, respectively. (G,H) EEG Power spectrum of wakefulness during 10 h (9:00–19:00) after the CNO injection in MPB-hM3Dq (G) and LPB-hM3Dq rats (H) . * P < 0.05, ** P < 0.01, compared with saline ( n = 8).
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Chemogenetic activation of MPB (but not LPB) neurons induces wakefulness. (A,B) Schematic diagram indicating the position of hM3Dq-mCherry protein was confined to the MPB (A) or LPB (B) . Superimposed mCherry expression areas in the MPB (A) and LPB (B) of 8 AAV-injected rats in each group, are shown in the right panels . Scale bars: 1 mm. (C,D) Typical examples of <t>EEG</t> <t>power</t> spectra, EEG, EMG, hypnogram, and relative magnitude changes in delta and theta power over 24 h for a rat expressing hM3Dq at MPB (C) or LPB neurons (D) when given CNO (0.3 mg/kg). (E,F) Time courses of wakefulness after administration of CNO (0.3 mg/kg) or saline in rats expressing hM3Dq in MPB (E) or LPB neurons (F) . The arrow indicates the time point of CNO or saline administration, and open and closed bars above the x -axis indicate light and dark periods, respectively. (G,H) EEG Power spectrum of wakefulness during 10 h (9:00–19:00) after the CNO injection in MPB-hM3Dq (G) and LPB-hM3Dq rats (H) . * P < 0.05, ** P < 0.01, compared with saline ( n = 8).
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Chemogenetic activation of MPB (but not LPB) neurons induces wakefulness. (A,B) Schematic diagram indicating the position of hM3Dq-mCherry protein was confined to the MPB (A) or LPB (B) . Superimposed mCherry expression areas in the MPB (A) and LPB (B) of 8 AAV-injected rats in each group, are shown in the right panels . Scale bars: 1 mm. (C,D) Typical examples of <t>EEG</t> <t>power</t> spectra, EEG, EMG, hypnogram, and relative magnitude changes in delta and theta power over 24 h for a rat expressing hM3Dq at MPB (C) or LPB neurons (D) when given CNO (0.3 mg/kg). (E,F) Time courses of wakefulness after administration of CNO (0.3 mg/kg) or saline in rats expressing hM3Dq in MPB (E) or LPB neurons (F) . The arrow indicates the time point of CNO or saline administration, and open and closed bars above the x -axis indicate light and dark periods, respectively. (G,H) EEG Power spectrum of wakefulness during 10 h (9:00–19:00) after the CNO injection in MPB-hM3Dq (G) and LPB-hM3Dq rats (H) . * P < 0.05, ** P < 0.01, compared with saline ( n = 8).
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Chemogenetic activation of MPB (but not LPB) neurons induces wakefulness. (A,B) Schematic diagram indicating the position of hM3Dq-mCherry protein was confined to the MPB (A) or LPB (B) . Superimposed mCherry expression areas in the MPB (A) and LPB (B) of 8 AAV-injected rats in each group, are shown in the right panels . Scale bars: 1 mm. (C,D) Typical examples of EEG power spectra, EEG, EMG, hypnogram, and relative magnitude changes in delta and theta power over 24 h for a rat expressing hM3Dq at MPB (C) or LPB neurons (D) when given CNO (0.3 mg/kg). (E,F) Time courses of wakefulness after administration of CNO (0.3 mg/kg) or saline in rats expressing hM3Dq in MPB (E) or LPB neurons (F) . The arrow indicates the time point of CNO or saline administration, and open and closed bars above the x -axis indicate light and dark periods, respectively. (G,H) EEG Power spectrum of wakefulness during 10 h (9:00–19:00) after the CNO injection in MPB-hM3Dq (G) and LPB-hM3Dq rats (H) . * P < 0.05, ** P < 0.01, compared with saline ( n = 8).

Journal: Frontiers in Neuroscience

Article Title: Medial Parabrachial Nucleus Is Essential in Controlling Wakefulness in Rats

doi: 10.3389/fnins.2021.645877

Figure Lengend Snippet: Chemogenetic activation of MPB (but not LPB) neurons induces wakefulness. (A,B) Schematic diagram indicating the position of hM3Dq-mCherry protein was confined to the MPB (A) or LPB (B) . Superimposed mCherry expression areas in the MPB (A) and LPB (B) of 8 AAV-injected rats in each group, are shown in the right panels . Scale bars: 1 mm. (C,D) Typical examples of EEG power spectra, EEG, EMG, hypnogram, and relative magnitude changes in delta and theta power over 24 h for a rat expressing hM3Dq at MPB (C) or LPB neurons (D) when given CNO (0.3 mg/kg). (E,F) Time courses of wakefulness after administration of CNO (0.3 mg/kg) or saline in rats expressing hM3Dq in MPB (E) or LPB neurons (F) . The arrow indicates the time point of CNO or saline administration, and open and closed bars above the x -axis indicate light and dark periods, respectively. (G,H) EEG Power spectrum of wakefulness during 10 h (9:00–19:00) after the CNO injection in MPB-hM3Dq (G) and LPB-hM3Dq rats (H) . * P < 0.05, ** P < 0.01, compared with saline ( n = 8).

Article Snippet: The bit map represents the EEG power spectra generated by MATLAB (The MathWorks, Inc., Massachusetts) ( ).

Techniques: Activation Assay, Expressing, Injection, Saline

Time course of sleep and power spectra starting 22 h after CNO treatment. (A) No bouts of NREM and REM sleep after the CNO treatment. Each circle represents the hourly mean amount of sleep. The horizontal open and closed bars on the x -axes indicate the 12-h light and 12-h dark periods, respectively. (B) EEG power spectra of NREM and REM sleep during the following day in rats injected with AAV-hSyn-hM3Dq-mCherry in MPB ( n = 8).

Journal: Frontiers in Neuroscience

Article Title: Medial Parabrachial Nucleus Is Essential in Controlling Wakefulness in Rats

doi: 10.3389/fnins.2021.645877

Figure Lengend Snippet: Time course of sleep and power spectra starting 22 h after CNO treatment. (A) No bouts of NREM and REM sleep after the CNO treatment. Each circle represents the hourly mean amount of sleep. The horizontal open and closed bars on the x -axes indicate the 12-h light and 12-h dark periods, respectively. (B) EEG power spectra of NREM and REM sleep during the following day in rats injected with AAV-hSyn-hM3Dq-mCherry in MPB ( n = 8).

Article Snippet: The bit map represents the EEG power spectra generated by MATLAB (The MathWorks, Inc., Massachusetts) ( ).

Techniques: Injection

Photostimulation of MPB glutamatergic neurons induced a rapid wakefulness response. (A) Coronal brain section with native mCherry fluorescence confirmed ChR2-mCherry was confined in the MPB. Scale bars: A = 200 μm, Ai = 100 μm, Aii = 20 μm. (B) The schematic diagram showing the cannula trace and the optic fibers targeting the MPB. (C) A single action potential (red trace) evoked by 5 ms blue light pulses, and double action potentials (gray trace) evoked by >10 ms blue light pulses from a ChR2-expressing neuron. (D) Firing traces from a ChR2-expressing MPB neuron evoked by 10 Hz (5 ms pulses) photostimulation. (E) Neuronal firing showing an effective entrainment up to 20 Hz optogenetic stimulation of MPB neurons. (F) Single-cell RT-PCR analysis of ChR2-expressing neurons in MPB indicates the band of 315 bp represents VGluT2. (G) EEG/EMG examples of the sleep to wakefulness transition following optogenetic stimulation in a rat with bilateral ChR2 (top) or mCherry (bottom) expression in MPB glutamatergic neurons. (H–J) The probability of sleep to wakefulness transition in the frequency range of 1–20 Hz in mCherry controls (H) , stimulating the MPB unilaterally (I) , and bilaterally (J) . (K,L) Typical examples of EEG power spectra, EEG, EMG, hypnogram, and relative magnitude changes in delta and theta power in a rat injected with AAV-CaMKIIα-ChR2-mCherry (K) or AAV-CaMKIIα-mCherry (L) bilaterally. (M,N) The time course (M) and total amount (N) of wakefulness during optogenetic stimulation. ** P < 0.01 compared with the mCherry groups ( n = 6).

Journal: Frontiers in Neuroscience

Article Title: Medial Parabrachial Nucleus Is Essential in Controlling Wakefulness in Rats

doi: 10.3389/fnins.2021.645877

Figure Lengend Snippet: Photostimulation of MPB glutamatergic neurons induced a rapid wakefulness response. (A) Coronal brain section with native mCherry fluorescence confirmed ChR2-mCherry was confined in the MPB. Scale bars: A = 200 μm, Ai = 100 μm, Aii = 20 μm. (B) The schematic diagram showing the cannula trace and the optic fibers targeting the MPB. (C) A single action potential (red trace) evoked by 5 ms blue light pulses, and double action potentials (gray trace) evoked by >10 ms blue light pulses from a ChR2-expressing neuron. (D) Firing traces from a ChR2-expressing MPB neuron evoked by 10 Hz (5 ms pulses) photostimulation. (E) Neuronal firing showing an effective entrainment up to 20 Hz optogenetic stimulation of MPB neurons. (F) Single-cell RT-PCR analysis of ChR2-expressing neurons in MPB indicates the band of 315 bp represents VGluT2. (G) EEG/EMG examples of the sleep to wakefulness transition following optogenetic stimulation in a rat with bilateral ChR2 (top) or mCherry (bottom) expression in MPB glutamatergic neurons. (H–J) The probability of sleep to wakefulness transition in the frequency range of 1–20 Hz in mCherry controls (H) , stimulating the MPB unilaterally (I) , and bilaterally (J) . (K,L) Typical examples of EEG power spectra, EEG, EMG, hypnogram, and relative magnitude changes in delta and theta power in a rat injected with AAV-CaMKIIα-ChR2-mCherry (K) or AAV-CaMKIIα-mCherry (L) bilaterally. (M,N) The time course (M) and total amount (N) of wakefulness during optogenetic stimulation. ** P < 0.01 compared with the mCherry groups ( n = 6).

Article Snippet: The bit map represents the EEG power spectra generated by MATLAB (The MathWorks, Inc., Massachusetts) ( ).

Techniques: Fluorescence, Expressing, Reverse Transcription Polymerase Chain Reaction, Injection

Chemogenetic inhibition of PB neurons decreased wakefulness in rats during the active period. (A) Brain section stained against eGFP to confirm IVMR expression in the PB. Injection sites were mapped on coronal atlas drawings at three different levels containing the PB. Superimposed eGFP expression areas in the PB of 6 AAV-injected rats are shown in the right panels. Scale bars: 1 mm. (B,C) Typical examples of EEG power spectra, EEG, EMG, hypnogram, and relative magnitude changes in delta and theta power over 24 h for the same rat given IVM (B) or IPA (C) . W, wakefulness; R, REM sleep; N, NREM sleep. (D) Time courses of wakefulness in rats after the administration of IVM (10 mg/kg) or IPA in IVMR rats and eGFP controls, respectively. (E) Amount of wakefulness during 10 h after IVM or IPA injection in IVMR and eGFP rats. * P < 0.05, ** P < 0.01, compared with the IPA treatment ( n = 6).

Journal: Frontiers in Neuroscience

Article Title: Medial Parabrachial Nucleus Is Essential in Controlling Wakefulness in Rats

doi: 10.3389/fnins.2021.645877

Figure Lengend Snippet: Chemogenetic inhibition of PB neurons decreased wakefulness in rats during the active period. (A) Brain section stained against eGFP to confirm IVMR expression in the PB. Injection sites were mapped on coronal atlas drawings at three different levels containing the PB. Superimposed eGFP expression areas in the PB of 6 AAV-injected rats are shown in the right panels. Scale bars: 1 mm. (B,C) Typical examples of EEG power spectra, EEG, EMG, hypnogram, and relative magnitude changes in delta and theta power over 24 h for the same rat given IVM (B) or IPA (C) . W, wakefulness; R, REM sleep; N, NREM sleep. (D) Time courses of wakefulness in rats after the administration of IVM (10 mg/kg) or IPA in IVMR rats and eGFP controls, respectively. (E) Amount of wakefulness during 10 h after IVM or IPA injection in IVMR and eGFP rats. * P < 0.05, ** P < 0.01, compared with the IPA treatment ( n = 6).

Article Snippet: The bit map represents the EEG power spectra generated by MATLAB (The MathWorks, Inc., Massachusetts) ( ).

Techniques: Inhibition, Staining, Expressing, Injection